Electroforesis capilar for the laboratory
A high-resolution separation technique that separates biomolecules inside fine capillaries by applying an electric field with temperature control. It can be coupled to optical detectors or to mass spectrometry.
electroforesis capilar guide
Characterization of monoclonal antibodies, glycan analysis, study of gene expression, DNA sequencing and fragment analysis, and quality control in the pharmaceutical, biotechnology and cell and gene therapy sectors.
How to choose electroforesis capilar
- It depends on the type of biomolecule to be analyzed
- The detection mode (absorbance, fluorescence or MS)
- Whether parallel separation (multiple capillaries) is required
- The sample volume and traceability. Specific values come from the catalog (Sanity)
Frequently asked questions about electroforesis capilar
When should capillary electrophoresis be chosen over HPLC?
When the separation depends on charge and size rather than hydrophobicity: proteins and their variants, glycans, nucleic acids and ions. CE resolves charge variants that chromatography cannot distinguish, with minimal sample volumes.
Why is it so widely used in biopharmaceuticals?
Because pharmacopeias and regulatory submissions require characterizing the purity and isoforms of therapeutic proteins: CE-SDS replaces SDS-PAGE with true quantification, and cIEF separates charge variants of monoclonal antibodies reproducibly.
What is CE-SDS and what advantage does it have over the traditional gel?
It is the capillary version of SDS-PAGE: it separates proteins by size with quantitative detection, full automation and electronic traceability, eliminating the variability and manual staining of gels.
Can it be coupled to mass spectrometry?
Yes: CE-MS combines the resolution of capillary separation with molecular identification, which is useful for glycans, peptides and in-depth characterization. Inolab represents Sciex, a leader in this technology.
Brands we represent in electroforesis capilar
Available electroforesis capilar models
- CESI 8000 Plus ESI-MS High-Performance SystemSciex
- GenomeLab GeXPSciex
- P/ACE MDQ PlusSciex
- PA 800 PlusSciex
More identificación molecular equipment
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