Cromatografía líquida for the laboratory
A liquid-phase separation technique that resolves the components of a mixture according to their affinity for a stationary phase and a mobile phase. It is usually coupled to optical detectors or to mass spectrometry.
cromatografía líquida guide
Separation, identification and quantification of compounds in pharmaceutical quality control, food and environmental analysis, proteomics, metabolomics and analytical chemistry in general.
How to choose cromatografía líquida
- It depends on the type of sample and compounds
- The detector required (optical or MS)
- Whether it is coupled to mass spectrometry
- The throughput
- The resolution needed and compatibility with existing methods. Specific values come from the catalog (Sanity)
Frequently asked questions about cromatografía líquida
HPLC or UHPLC: which is better?
UHPLC operates at higher pressures with sub-2 µm particles: faster runs, better resolution and less solvent. If you have inherited validated HPLC methods, migrating means revalidating; many laboratories choose instruments compatible with both regimes.
Which detector do I need?
UV/DAD covers most routine assays; fluorescence provides sensitivity for trace levels; and coupling to mass spectrometry (LC-MS) delivers unambiguous identification and quantification in complex matrices. The choice depends on the analyte and on the detection limit required.
What is needed for a regulated (pharmaceutical) environment?
Software with data integrity (21 CFR Part 11), IQ/OQ/PQ qualification of the system, traceable columns and standards, and a preventive maintenance program. The entire workflow must withstand an audit of raw data.
Why couple liquid chromatography to mass spectrometry?
Because retention alone does not identify: the spectrometer confirms molecular identity and makes it possible to quantify in the presence of interferences, which is key in bioanalysis, impurities and peptides. Inolab represents Sciex in Mexico for these solutions.
Brands we represent in cromatografía líquida
Available cromatografía líquida models
More identificación molecular equipment
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