Electrofisiología for the laboratory
A set of techniques for recording the electrical activity of cells and tissues, especially the study of ion channels by patch-clamp. It uses very low-noise amplifiers, digitizers and acquisition software.
electrofisiología guide
Neurophysiology, the study of ion channels, research in biotechnology and molecular biology, and evaluation of electrical activity in excitable cells.
How to choose electrofisiología
- It depends on the recording technique (whole-cell, single-channel, extracellular)
- The acceptable noise level
- The clamp modes (voltage or current)
- Line interference cancellation and integration with the analysis software. Specific values come from the catalog (Sanity)
Frequently asked questions about electrofisiología
What do I need to set up a patch-clamp rig?
The core elements: a low-noise amplifier and digitizer, acquisition and analysis software, micromanipulators, a suitable microscope, an anti-vibration table and a Faraday cage. The quality of the amplifier sets the ceiling for your recordings.
What is the difference between voltage-clamp and current-clamp?
In voltage-clamp you hold the membrane potential and measure currents (ideal for ion channels); in current-clamp you inject current and observe the voltage (the firing behavior of the neuron). A good amplifier handles both modes.
Why is noise so critical and how is it controlled?
The currents measured are in the picoampere range: any electrical noise or vibration masks them. It is controlled with shielding (Faraday cage), well-designed grounding, an anti-vibration table and careful configuration of the rig — part of the support we provide during installation.
Is the recording compatible with cardiac safety studies (hERG)?
Yes: manual patch-clamp remains the gold standard for characterizing the hERG channel and other cardiac safety targets required by the ICH S7B guidelines.
Brands we represent in electrofisiología
Available electrofisiología models
- Axoclamp 900AMolecular Devices
- Axon Digidata 1550B LowMolecular Devices
- Axopatch 200BMolecular Devices
- MultiClamp 700BMolecular Devices
More biotecnología y biología molecular equipment
- Citometría de flujo
- Lectores de microplacas
- Microarreglos
- Cribado y selección de clones
- Analizadores ELISpot/FluoroSpot
Not sure which one you need? Ask Inolab's AI advisor and get the right electrofisiología recommendation for your application, with real specifications and a quote from a specialist. Back to the advisor ↑