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Lectores de microplacas for the laboratory

A microplate reader is an instrument designed to measure absorbance, fluorescence or luminescence signals in multiple samples held simultaneously in the wells of a standardized plate, typically with 6, 12, 24, 48, 96, 384 or 1536 wells. Its function is to convert an optical property of each well into a quantitative value that correlates with the concentration, activity or biological state of the analyte under study. All the wells of the plate undergo the same protocol, at the same time and in the same thermal and optical environment, which makes it possible to compare hundreds of experimental conditions within a single assay with a single calibration curve. It is this characteristic that makes the instrument the central platform for high-throughput screening and for plate-format immunoassays.

lectores de microplacas guide

Microplate readers are widely used in all variants of ELISA assays, endotoxin detection, quantification of proteins and nucleic acids, viability and cytotoxicity assays, proliferation, reporter assays (luciferase, GFP), FRET and GPCR assays. They are important instruments in the fields of diagnostics, immunoassays, cell biology, molecular biology, microbiology and pharmaceutical quality control.

How to choose lectores de microplacas

  • To choose the right instrument
  • It is important to consider which detection mode you need — absorbance
  • Fluorescence
  • Luminescence or whether you require multimode analysis — as well as the plate format and density
  • Whether temperature control or injectors are required
  • The sensitivity of the detector and the throughput

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