Molecular Devices·Electrofisiología

Axopatch 200B

Axopatch 200B — instrumento de electrofisiología de Molecular Devices (Open-circuit noise: 0.13 pA rms (10 kHz)).

0.13 pA rms (10 kHz)Nivel de ruido

0.145 pA RMSNivel de ruido

up to 100 kHzRecording bandwidth

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Descripción

Conoce el equipo

Axopatch 200B es un(a) instrumento de electrofisiología de Molecular Devices, indicado para registro de canales iónicos, patch clamp, estudios de membrana celular. Especificaciones destacadas: Open-circuit noise: 0.13 pA rms (10 kHz); Noise with patch-pipette holder attached: 0.145 pA RMS.

Aplicaciones ideales

Resuelve tu aplicación

Registro de canales iónicos

Patch clamp

Estudios de membrana celular

Especificaciones técnicas

Especificaciones
del Axopatch 200B

Open-circuit noise: 0.13 pA rms (10 kHz)
Noise with patch-pipette holder attached: 0.145 pA RMS
Recording bandwidth: up to 100 kHz
Input circuitry cooling temperature: -15 °C typical
Gain (b=1): 1 mV/pA
Gain (b=0.1): 0.1 mV/pA
Feedback element Patch: 1 pF
Feedback element Whole Cell b=1: 500 MΩ in parallel with 1 pF
Feedback element Whole Cell b=0.1: 50 MΩ in parallel with 1 pF
Pipette-capacitance-compensation injection capacitor: 1 pF
Whole-cell-capacitance-compensation injection capacitor Whole Cell b=1: 5 pF
Whole-cell-capacitance-compensation injection capacitor Whole Cell b=0.1: 50 pF
Bandwidth Internal patch mode: 140 kHz
Bandwidth Internal whole-cell mode: 70 kHz
Bandwidth Max. external: 100 kHz
Capacitative load stability: 1000 pF
Max. Instrument Noise Without Holder Patch Line Frequency & Harmonics: 0.005 pAp-p
Max. Instrument Noise Without Holder Patch 0.1–100 Hz: 0.030 pAp-p
Max. Instrument Noise Without Holder Patch 0.1–1 kHz: 0.015 pArms
Max. Instrument Noise Without Holder Patch 0.1–5 kHz: 0.060 pArms
Max. Instrument Noise Without Holder Patch 0.1–10 kHz: 0.130 pArms
Max. Instrument Noise Without Holder Whole Cell b=1 Line Frequency & Harmonics: 0.005 pAp-p
Max. Instrument Noise Without Holder Whole Cell b=1 0.1–100 Hz: 0.50 pAp-p
Max. Instrument Noise Without Holder Whole Cell b=1 0.1–1 kHz: 0.25 pArms
Max. Instrument Noise Without Holder Whole Cell b=1 0.1–5 kHz: 0.65 pArms
Max. Instrument Noise Without Holder Whole Cell b=1 0.1–10 kHz: 1.10 pArms
Max. Instrument Noise Without Holder Whole Cell b=0.1 Line Frequency & Harmonics: 0.005 pAp-p
Max. Instrument Noise Without Holder Whole Cell b=0.1 0.1–100 Hz: 1.6 pAp-p
Max. Instrument Noise Without Holder Whole Cell b=0.1 0.1–1 kHz: 0.75 pArms
Max. Instrument Noise Without Holder Whole Cell b=0.1 0.1–5 kHz: 1.65 pArms
Max. Instrument Noise Without Holder Whole Cell b=0.1 0.1–10 kHz: 3.0 pArms
Max. Instrument Noise With Holder 0.1–10 kHz: 0.145 pArms
Max. Instrument Noise With Holder Whole Cell b=1 0.1–10 kHz: 1.10 pArms
Max. Instrument Noise With Holder Whole Cell b=0.1 0.1–10 kHz: 3.0 pArms
Total reset time: 50 μs ±10%
Reset transients at 100 Hz: ±0.25 pA
Reset transients at 1 kHz: ±0.5 pA
Reset transients at 10 kHz: ±2 pA
Pipette offset potential: ±250 mV
Zap pulse voltage: +1.3 V
Seal Test voltage clamp pulse: 5 mV
Seal Test current clamp pulse b=1: 50 pA
Seal Test current clamp pulse b=0.1: 500 pA
Whole-Cell Capacitance compensation b=1: up to 100 pF
Whole-Cell Capacitance compensation b=0.1: up to 1000 pF
Capacitance Dithering b=1: 100 fF
Capacitance Dithering b=0.1: 1 pF
DR-1 Resistance Dither resistor: 500 kΩ
Output swing: ±10 V
Output gain settings range: 1000 fold
Low-pass filter settings: 1 kHz up to 100 kHz
I-Clamp Fast mode rise time: 10 μs
Pipette resistance for I-Clamp Fast mode: above 10 MΩ
Pipette resistance for I-Clamp Normal mode: above 1 MΩ
HL-U pipette holder OD acceptance: 1.0–1.7 mm
HL-U pipette holder post OD: 1.0 mm